flowType: Phenotyping Flow Cytometry Assays
نویسنده
چکیده
2 Introduction This document demonstrates the functionality of the flowType package for phenotyping FCM assays. flowType uses a simple threshold, Kmeans, flowMeans or flowClust to partition every channel to a positive and a negative cell population. These partitions are then combined to generate a set of multi-dimensional phenotypes. For more details on how this package can be used in a complete analysis pipeline, please refer to the manuscript that describes analysis of 466 HIV patients [1]. A Sweave example is available at http://www.terryfoxlab. ca/flowsite/flowType.
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Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays
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